elisa analysis  (R&D Systems)

Journal: bioRxiv

Article Title: Impaired lipoprotein secretion by APOE4 leads to lysosomal and mitochondrial dysfunction in human microglia

doi: 10.64898/2026.05.12.724612

Figure Lengend Snippet: (A) Schematic of donors divided by APOE haplotypes (APOE23 n = 5, APOE33 n = 6, APOE34 n = 7, APOE44 n = 4). (B) Density plot of scRNAseq UMAP per APOE haplotype displaying distribution across identified clusters. (C) Distribution and proportion of cells across different cell states, separated by APOE4-and APOE4+. Each dot represents an individual line. Unpaired t-test, two-tailed, mean ± SEM. (D) Percentage of BHLHE40-/41- microglia in ROSMAP data set, split by APOE haplotypes and diagnoses. Each dot represents an individual donor (CTRL APOE23 n = 13, AD APOE23 n = 6, CTRL APOE33 n = 50, AD APOE33 n = 48, AD APOE44 n = 6). Chi-squared test, mean ± SEM. (E) Compass analysis of metabolic pathways in APOE44 iMG vs APOE33 iMG in G0 phase cells. Select terms shown. Wilcoxon signed-rank test, significance set to padj < 0.05. (F) Enrichment analysis of downregulated genes in APOE44 compared to other haplotypes (Group 1, N=186 genes). (G) Enrichment analysis of upregulated genes in APOE44 compared to other haplotypes (Group 4, N=80 genes). Created using Metascape . (C-D) *p < 0.05; ns, non-significant See also .

Article Snippet: After an additional 24 hours, supernatant and cells were collected for APOE ELISA analysis (Mabtech, 3712-1HP-1) using manufacturer’s instructions.

Techniques: Two Tailed Test

Journal: bioRxiv

Article Title: Impaired lipoprotein secretion by APOE4 leads to lysosomal and mitochondrial dysfunction in human microglia

doi: 10.64898/2026.05.12.724612

Figure Lengend Snippet: (A) Distribution and proportion of cells across different cell states, separated by APOE genotype. Each dot represents an individual line. Unpaired t-test, two-tailed, mean ± SEM. (B) Proportion of female cells across FDAMic cluster, separated by APOE4- and APOE4+. Each dot represents an individual line. One way ANOVA, mean ± SEM. (C) Compass analysis of metabolic pathways in APOE44 vs APOE33 lines in G0 phase cells. Wilcoxon signed-rank test, significance set to padj < 0.05 (D) DEGs across APOE haplotypes reveal 4 specific patterns of expression. (E) Expression of genes GM2A, SCARB2 and RPL8 across APOE haplotypes. Each dot represents an individual line. One way ANOVA, mean ± SEM. (F) Venn diagram of number of DEGs with Likelihood Ratio Test (padj < 0.05) separated by sex and analyzed by APOE haplotype. (G,H) Enrichment analyses of downregulated and upregulated genes in (G) male and (H) female lines across APOE haplotype. Significance set to Wald |Log2FC(APOE44 vs APOE33)| > 0, LRT padj < 0.05. Created using Metascape . (I) Volcano plots of ROSMAP APOE44 microglia vs APOE33 microglia. Down sampled to n = 8 for each group. Likelihood Ratio Test, p-values were corrected using the Benjamini-Hochberg correction for multiple testing, significance set to padj < 0.05, |Log2FC cutoff| > 0. (J,K) Enrichment analyses of (J) downregulated and (K) upregulated genes comparing ROSMAP APOE44 microglia vs APOE33 microglia. Significance set to p < 0.05, |Log2FC cutoff| > 0. Created using Metascape . (L) Log2FC changes of genes comparing APOE44 to APOE33 ROSMAP microglia against APOE44 and APOE33 donor iMG. Genes with padj (LRT) < 0.05 for the donor iMG are shown. Log2FCs from Wald analysis are shown for donor iMG. (M-N) Enrichment analyses of (M) downregulated genes in both datasets and (N) upregulated genes in both datasets. Significance set to padj (LRT) < 0.05 for donor iMG. |Log2FC cutoff| > 0 in ROSMAP and donor iMG dataset. Created using Metascape . *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001

Article Snippet: After an additional 24 hours, supernatant and cells were collected for APOE ELISA analysis (Mabtech, 3712-1HP-1) using manufacturer’s instructions.

Techniques: Two Tailed Test, Expressing

Journal: bioRxiv

Article Title: Impaired lipoprotein secretion by APOE4 leads to lysosomal and mitochondrial dysfunction in human microglia

doi: 10.64898/2026.05.12.724612

Figure Lengend Snippet: (A) Seahorse Mito Stress Test kit. Proton leak, spare capacity and non-mito oxygen consumption normalized to confluency of wells, comparing APOE33 and APOE44 iMG. Each dot represents a replicate well. (B) Expression of LDHA across different APOE haplotypes in the donor iMG dataset. Each dot represents an individual line. One way ANOVA, mean ± SEM. (C) Relative abundance of lactate after tracing with [U- 13 C 6 ] glucose. (D) Labeled citrate, malate, glutamate, and aspartate after [U- 13 C 6 ] glucose tracing. Two-way ANOVA, Šídák’s multiple comparisons test, mean ± SEM. (E) TMRE staining and quantification of mean intensity per puncta across APOE33 and APOE44 iMG (n = 3 well x 10 frames per line). Scale bar = 50µm. (F) Total glutathione measured from cell lysates using kit (MedChem, HY-K0311). (G) SOD2, SQOR, and NQO1 protein levels across APOE33 and APOE44 iMG. Unpaired t-test, two-tailed, mean ± SEM, corrected with Benjamini-Hochberg (n = 3 wells each). (H) Expression of ALOX5AP across different APOE haplotypes in the donor iMG dataset. Each dot represents an individual line. One way ANOVA, mean ± SEM. (I) Percentage of ALOX5AP+ microglia in the ROSMAP dataset across different APOE haplotypes and diagnoses. Chi-squared test (CTRL APOE23 n = 13, AD APOE23 n = 6, CTRL APOE33 n = 50, AD APOE33 n = 48, AD APOE44 n = 6). (A, C, E-F) Unpaired t-test, two-tailed, mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. ns, non-significant

Article Snippet: After an additional 24 hours, supernatant and cells were collected for APOE ELISA analysis (Mabtech, 3712-1HP-1) using manufacturer’s instructions.

Techniques: Expressing, Labeling, Staining, Two Tailed Test

Journal: bioRxiv

Article Title: Impaired lipoprotein secretion by APOE4 leads to lysosomal and mitochondrial dysfunction in human microglia

doi: 10.64898/2026.05.12.724612

Figure Lengend Snippet: (A) Module scores created using DEGs from Haney et al. plotted on our UMAP space . Left represents upregulated genes in lipid high APOE44 iMG, right represents downregulated genes in lipid high APOE44 iMG. (B) Density UMAP of expression of CDKN1A (p21) and CDKN2A (p16). (C) Distribution and proportion of cells in G2/M and G0 phase, separated by APOE4- and APOE4+. Each dot represents an individual line. Mann-Whitney U-test, two-tailed, mean ± SEM. (D) Representative image of p16 staining with DAPI on iMG. Scale bar = 130µm. Arrows point toward examples of nuclei with larger areas. (E) ImageJ analysis of nuclei size measuring area of DAPI from p16- and p16+ iMG. Each dot represents a different cell (p16- n = 10, p16+ n = 10). (F) Schematic of using Vybrant DyeCycle Violet Dye with verapamil to stain live nuclei in APOE44 iMG to collect 2N (G0) and 4N (G2) cells. (G) Using a plate reader, G0 and G2 cells that were collected were stained again with DyeCycle and verapamil 72 hours later. G2 is normalized to G0 in each run and cell count. Each dot represents a replicate well. (H) Relative abundance of cholesterol esters between G0 and G2 cells normalized to G0. (I) p16+ microglia in ROSMAP dataset across different APOE haplotypes and diagnoses. CTRL APOE33 n = 50, AD APOE33 n = 48, AD APOE44 n = 6. (E, G, H) Unpaired t-test, two-tailed, mean ± SEM. (I) Chi-squared test, mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. ns, non-significant See also .

Article Snippet: After an additional 24 hours, supernatant and cells were collected for APOE ELISA analysis (Mabtech, 3712-1HP-1) using manufacturer’s instructions.

Techniques: Expressing, MANN-WHITNEY, Two Tailed Test, Staining, Cell Characterization

Journal: bioRxiv

Article Title: Impaired lipoprotein secretion by APOE4 leads to lysosomal and mitochondrial dysfunction in human microglia

doi: 10.64898/2026.05.12.724612

Figure Lengend Snippet: A) Schematic of transwell setup of induced neurons (iNs) with APOE33 and APOE44 iMG. (B) Violin plot of lipidomics of APOE33 and APOE44 iMG cocultured with iNs or alone. Each dot represents a different lipid species within the iMG lysates. (C) Free cholesterol compared between APOE33 and 44 iMG, cocultured with iNs or alone. (D) Sum of lipid species in different lipid classes compared between iNs cocultured with APOE33 iMG vs with APOE44 iMG. (E) Boxplot of ZScores of lipid species from iNs cocultured with APOE33 iMG and APOE44 iMG. (F) PSD95+/Synapsin+ puncta and (G) Syn+ puncta quantified in iNs cocultured with either APOE33 or APOE44 iMG, normalized to area of Tuj1. Plotted as fold change differences normalized to iN cocultured with APOE33 iMG. (H) APOE ELISA of supernatant from APOE33 and APOE44 iMG (n = 5 wells each). (I) HDL-like particles measured in supernatant and normalized to protein of lysate (n = 4 wells each). (J) APOE ELISA of supernatant from UCI5 APOE33 and UCI5 APOE44 iMG (n = 4 wells each). (K) BODIPY-493/503 quantified as mean number of puncta per cell per frame across UCI5 APOE33 and APOE44 iMG (n = 3 wells x 10 frames per group). (L) MMP measured with JC-1 dye comparing UCI5 APOE33 and UCI5 APOE44 iMG (n = 4 wells each). On a Glomax plate reader, red was measured at an excitation of 520 nm and green at an excitation of 475 nm. (M) Late endosomal and lysosomal acidity measured ratiometrically by dextran polymers labeled with the pH sensor ApHID and Alexa 647 (pH-independent) across UCI5 APOE33 and APOE44 iMG untreated and treated with organoid debris (n=3 wells each, 25 frames per well). (N) APOE ELISA of supernatant from 8 donor lines, 4 APOE33 lines and 4 APOE44 lines (n = 4 wells each). (O) MMP measured with JC-1 dye comparing 6 donor lines, 3 APOE33 lines and 3 APOE44 lines (n = 4 wells each). On a Glomax plate reader, red was measured at an excitation of 520 nm and green at an excitation of 475 nm. (D-G, I-M) Unpaired t-test, two-tailed, mean ± SEM. (C, H) One way ANOVA, mean ± SEM. (N, O) Nested t-test, two-tailed, mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 See also .

Article Snippet: After an additional 24 hours, supernatant and cells were collected for APOE ELISA analysis (Mabtech, 3712-1HP-1) using manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Labeling, Two Tailed Test

Journal: bioRxiv

Article Title: Impaired lipoprotein secretion by APOE4 leads to lysosomal and mitochondrial dysfunction in human microglia

doi: 10.64898/2026.05.12.724612

Figure Lengend Snippet: (A) Sum of lipid species separated by class, plotted across APOE33 and APOE44 iMG cocultured with iNs or alone (n = 3 wells each). (B) Staining of Iba1 and Tuj1 (left); Tuj1, PSD95 and Synapsin (middle) and segmentation (right) in APOE33 iMG + iN8011 (top) and APOE44 iMG + iN8011 (bottom). Scale bar = 50µm. (C) APOE ELISA of cell lysates from KOLF APOE33(DMSO), APOE44(DMSO) and APOE44(GW) normalized to total cell lysate protein (n = 5 wells each). (D) APOE mRNA levels across KOLF APOE33(DMSO), APOE44(DMSO) and APOE44(GW) (n = 2 wells each). Wald test, corrected using the Benjamini-Hochberg correction for multiple testing, mean ± SEM. (E) APOE ELISA of cell lysates from UCI5 APOE33 and APOE44 iMG normalized to total cell lysate protein (n = 4 wells each). Unpaired t-test, two-tailed, mean ± SEM. (F-G) Enrichment analyses of (F) upregulated and (G) downregulated proteins in UCI5 APOE44 vs APOE33 iMG proteomic dataset (n = 3 wells each). Created using Metascape . Unpaired t-test, two-tailed, mean ± SEM, corrected with Benjamini-Hochberg. Significance set to padj < 0.05, |Log2FC cutoff| > 0.5. (H) Gene expression Log2FC for APOE44 vs APOE33 in KOLF iMG compared with corresponding changes in UCI5 iMG. Functional enrichment of overlapping genes. (I) Protein expression Log2FC for APOE44 vs APOE33 in KOLF iMG compared with corresponding changes in UCI5 iMG. Functional enrichment of overlapping genes. (J) Transcriptomic and proteomic Log2FC comparison of APOE44 vs APOE33 in KOLF iMG. Functional enrichment of overlapping genes. (K) Transcriptomic and proteomic Log2FC comparison of APOE44 vs APOE33 in UCI5 iMG. Functional enrichment of overlapping genes. (A, C) One way ANOVA, mean ± SEM. (H-K) Enrichment analyses of overlapping upregulated and downregulated proteins. Significance set to padj < 0.05. Created using Metascape . *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001

Article Snippet: After an additional 24 hours, supernatant and cells were collected for APOE ELISA analysis (Mabtech, 3712-1HP-1) using manufacturer’s instructions.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Gene Expression, Functional Assay, Expressing, Comparison

Journal: Virus Research

Article Title: RAB25 promotes hepatitis B virus-induced liver fibrosis progression through activation of the PI3K/AKT signaling pathway

doi: 10.1016/j.virusres.2026.199698

Figure Lengend Snippet: RAB25 is upregulated in activated HSCs induced by HBV-associated hepatocellular carcinoma cells. A: ELISA assay was applied to detect TGF-β1 level in HepG2 and HepG2.2.15 cells. ** P < 0.01, vs. the HepG2 group. B-D: LX-2 cells were subjected to 24-h co-culture with HepG2.2.15 and HepG2 cells in Transwell chambers, respectively, and then collected for CCK-8 (B), cell colony formation assay (C) and scratch assay (D) were carried out respectively to examine the changes in HSCs viability, proliferation, and migration. E: The expression levels of fibrosis markers (α-SMA and Collagen I), matrix remodeling factor MMP2, and proliferation-associated protein PCNA in HSCs were detected using Western blot. F: AQP1, CXCL10, CCL20, SOX9, and RAB25 mRNA levels in HSCs were detected using qRT-PCR. F: RAB25 protein level in HSCs was detected using Western blot. N=3 (biological replicates). * P < 0.05, ** P < 0.01, *** P < 0.001, vs. the HepG2+ HSC group.

Article Snippet: Serum samples were collected from all participants after overnight fasting and stored at −80°C for subsequent serum RAB25 expression analysis by ELISA (Human RAB25 ELISA Kit, SLD4057Hu, Sunlong Biotech, Hangzhou, China).

Techniques: Enzyme-linked Immunosorbent Assay, Co-Culture Assay, CCK-8 Assay, Colony Assay, Wound Healing Assay, Migration, Expressing, Western Blot, Quantitative RT-PCR

Journal: Virus Research

Article Title: RAB25 promotes hepatitis B virus-induced liver fibrosis progression through activation of the PI3K/AKT signaling pathway

doi: 10.1016/j.virusres.2026.199698

Figure Lengend Snippet: Silencing RAB25 inhibits TGF-β1-induced activation of HSCs. A-B: si-RAB25 knockdown efficiency was detected using qRT-PCR (A) and Western blot (B), ** P < 0.01, vs. the si-NC group. C-F: The si-RAB25 and its control si-NC were transfected into cells and cultured for 48 h. HSCs were then subjected to 24-h stimulation with 4 ng/ml TGF-β1. CCK-8 (C), cell colony formation assay (D), and scratch assay (E) were carried out, respectively, to detect changes in viability, proliferation, and migration ability of HSCs in different treatment groups. F: Using Western blot, the expressions of fibrosis markers (α-SMA and Collagen I), matrix remodeling factor MMP2, and proliferation-associated protein PCNA in HSCs from treatment groups were detected. N=3 (biological replicates). ** P < 0.01, vs. Blank group; ## P < 0.01, vs. the TGF-β1+si-NC group.

Article Snippet: Serum samples were collected from all participants after overnight fasting and stored at −80°C for subsequent serum RAB25 expression analysis by ELISA (Human RAB25 ELISA Kit, SLD4057Hu, Sunlong Biotech, Hangzhou, China).

Techniques: Activation Assay, Knockdown, Quantitative RT-PCR, Western Blot, Control, Transfection, Cell Culture, CCK-8 Assay, Colony Assay, Wound Healing Assay, Migration

Journal: Virus Research

Article Title: RAB25 promotes hepatitis B virus-induced liver fibrosis progression through activation of the PI3K/AKT signaling pathway

doi: 10.1016/j.virusres.2026.199698

Figure Lengend Snippet: Overexpression of RAB25 activates TGF-β1-induced activation of HSCs. A-B: The transfection efficiency of RAB25 overexpression to HSCs was detected using qRT-PCR (A) and Western blot (B), ** P < 0.01, vs. the NC group. C-F: The RAB25 overexpression and its negative control were transfected into cells and cultured for 48 h. HSCs were subsequently subjected to 24-h stimulation with 4 ng/ml TGF-β1. CCK-8 (C), cell colony formation assay (D), and scratch assay (E) were implemented, respectively, to detect changes in viability, proliferation, and migration ability of HSCs in different treatment groups. F: The expressions of α-SMA and Collagen I, MMP2, and PCNA in HSCs from treatment groups were detected by Western blot. N=3 (biological replicates). ** P < 0.01, vs. Blank group; ## P < 0.01, vs. the TGF-β1+NC group.

Article Snippet: Serum samples were collected from all participants after overnight fasting and stored at −80°C for subsequent serum RAB25 expression analysis by ELISA (Human RAB25 ELISA Kit, SLD4057Hu, Sunlong Biotech, Hangzhou, China).

Techniques: Over Expression, Activation Assay, Transfection, Quantitative RT-PCR, Western Blot, Negative Control, Cell Culture, CCK-8 Assay, Colony Assay, Wound Healing Assay, Migration

Journal: Virus Research

Article Title: RAB25 promotes hepatitis B virus-induced liver fibrosis progression through activation of the PI3K/AKT signaling pathway

doi: 10.1016/j.virusres.2026.199698

Figure Lengend Snippet: Silencing RAB25 inhibits HSC activation induced by HBV-associated hepatocellular carcinoma cells. The RAB25 knockdown vector or the control vector was transfected into LX-2 and cultured for 48 h. Next, the transfected LX-2 was subjected to 24-h co-culture with HepG2.2.15 and HepG2, respectively, and the LX-2 cells were harvested for the subsequent experiments. A-C: The changes in viability, proliferation, and migration ability of HSCs in different treatment groups were detected using CCK-8 (A), cell colony formation assay (B), and scratch assay (C), respectively. D: Using Western blot, the expression levels of fibrosis markers (α-SMA and Collagen I), matrix remodeling factor MMP2, and proliferation-associated protein PCNA in HSCs in different treatment groups were detected. N=3 (biological replicates). ** P < 0.01, vs. HepG2+HSC group; ## P < 0.01, vs. HepG2.2.15+HSC+si-NC group.

Article Snippet: Serum samples were collected from all participants after overnight fasting and stored at −80°C for subsequent serum RAB25 expression analysis by ELISA (Human RAB25 ELISA Kit, SLD4057Hu, Sunlong Biotech, Hangzhou, China).

Techniques: Activation Assay, Knockdown, Plasmid Preparation, Control, Transfection, Cell Culture, Co-Culture Assay, Migration, CCK-8 Assay, Colony Assay, Wound Healing Assay, Western Blot, Expressing

Journal: Virus Research

Article Title: RAB25 promotes hepatitis B virus-induced liver fibrosis progression through activation of the PI3K/AKT signaling pathway

doi: 10.1016/j.virusres.2026.199698

Figure Lengend Snippet: Effects of RAB25 overexpression on HSCs activation induced by HBV-associated hepatocellular carcinoma cells. The RAB25 overexpression vector or the control vector was transfected into LX-2 and cultured for 48 h. Next, the transfected LX-2 was subjected to 24-h co-culture with HepG2.2.15 and HepG2, respectively, and the LX-2 cells were harvested for the subsequent experiments. A-C: The changes in viability, proliferation, and migration ability of HSCs in different treatment groups were explored using CCK-8 (A), cell colony formation assay (B), and scratch assay (C), respectively. D: Using Western blot, the expression levels of α-SMA, Collagen I, MMP2 and PCNA in HSCs in different treatment groups were detected. N=3 (biological replicates). ** P < 0.01, vs. HepG2+HSC group; ## P < 0.01, vs. HepG2.2.15+HSC+NC group.

Article Snippet: Serum samples were collected from all participants after overnight fasting and stored at −80°C for subsequent serum RAB25 expression analysis by ELISA (Human RAB25 ELISA Kit, SLD4057Hu, Sunlong Biotech, Hangzhou, China).

Techniques: Over Expression, Activation Assay, Plasmid Preparation, Control, Transfection, Cell Culture, Co-Culture Assay, Migration, CCK-8 Assay, Colony Assay, Wound Healing Assay, Western Blot, Expressing

Journal: Virus Research

Article Title: RAB25 promotes hepatitis B virus-induced liver fibrosis progression through activation of the PI3K/AKT signaling pathway

doi: 10.1016/j.virusres.2026.199698

Figure Lengend Snippet: RAB25 promotes PI3K-AKT signaling activation in HBV-associated hepatocellular carcinoma cell-induced HSCs activation. A: si-RAB25#1 and its control product were transfected into LX-2 and cultured for 48 h. Next, the transfected LX-2 was subjected to 24-h co-culture with HepG2.2.15 and HepG2, respectively. The total and phosphorylated PI3K and AKT level in HSCs from all groups was then detected using Western blot. ** P < 0.01, vs. HepG2+HSC group; ## P < 0.01, vs. the HepG2.2.15+HSC+si-NC group. B: RAB25 overexpression vector or the control vector was transfected into LX-2 and cultured for 48 h. Next, the transfected LX-2 was subjected to 24-h co-culture with HepG2.2.15 and HepG2, respectively. Then, the total and phosphorylated PI3K and AKT levels in HSCs from all groups were detected using Western blot. N=3 (biological replicates). ** P < 0.01, vs. HepG2+HSC group; ## P < 0.01, vs. the HepG2.2.15+HSC+NC group.

Article Snippet: Serum samples were collected from all participants after overnight fasting and stored at −80°C for subsequent serum RAB25 expression analysis by ELISA (Human RAB25 ELISA Kit, SLD4057Hu, Sunlong Biotech, Hangzhou, China).

Techniques: Activation Assay, Control, Transfection, Cell Culture, Co-Culture Assay, Western Blot, Over Expression, Plasmid Preparation

Journal: Virus Research

Article Title: RAB25 promotes hepatitis B virus-induced liver fibrosis progression through activation of the PI3K/AKT signaling pathway

doi: 10.1016/j.virusres.2026.199698

Figure Lengend Snippet: Rescue of RAB25-mediated HSCs activation by PI3K/AKT inhibition. HepG2.2.15 cells were co‑cultured with LX‑2 hepatic stellate cells transiently transfected with a RAB25 overexpression vector; cells were treated with either dimethyl sulfoxide (DMSO) as vehicle control or the selective PI3K inhibitor LY294002 at a concentration of 10 μM for 24 h. Then, treated HSCs were examined for A: the total and phosphorylated PI3K and AKT level using Western blot; B: cell viability using CCK-8; C: cell proliferation by colony formation assay; D: cell migration through scratch assay; E: the expression levels of α-SMA, Collagen I, MMP2, and PCNA using Western blot. N=3 (biological replicates). ** P < 0.01, vs. HepG2.2.15+HSC-RAB25+DMSO group.

Article Snippet: Serum samples were collected from all participants after overnight fasting and stored at −80°C for subsequent serum RAB25 expression analysis by ELISA (Human RAB25 ELISA Kit, SLD4057Hu, Sunlong Biotech, Hangzhou, China).

Techniques: Activation Assay, Inhibition, Transfection, Over Expression, Plasmid Preparation, Control, Concentration Assay, Western Blot, CCK-8 Assay, Colony Assay, Migration, Wound Healing Assay, Expressing